Regeneration of Salvia sclarea via organogenesis

The present work provides a system for regeneration of clary sage (Salvia s clarea L.) via organogenesis using plant tissue culture techniques in a mul tistage culturing medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) (9.05-181.00 mu M). A higher frequency of organogenic tissue initiation was obtained from immature zygotic embryo cotyledons (IZEC) 2-3 wk after polli nation on the medium supplemented with 9.05 mu M 2,4-D. The organogenic tis sues were then proliferated on media containing both indole-3-acetic acid ( IAA) and 6-benzylaminopurine (BA). Organogenic lines were established via s election, isolation and continuous subculture of organogenic tissues on a m edium containing 22.19 mu M BA and 2.85 mu M IAA. Shoots were regenerated f rom both the proliferated tissues and IZEC, and propagated in the presence of IAA or alpha-naphthaleneacetic acid (NAA). BA and gibberellic acid (GA(3 )). Although roots were induced from regenerated shoots on the media contai ning a low concentration of IAA, IBA (0.98 mu M) in combination with desicc ation of regenerated shoots with a stem similar to 10 mm in length promoted more and stronger root formation. After the root system was well establish ed (20 mm in length), the regenerated plants were transferred to soil in pl astic pots for further growth and production of R1 seeds in the greenhouse.