Growth of Mycobacterium tuberculosis in a defined medium is very restricted by acid pH and Mg2+ levels

Mycobacterium tuberculosis grows within the phagocytic vacuoles of macropha ges, where it encounters a moderately acidic and possibly nutrient-restrict ed environment. Other mycobacterial species encounter acidic conditions in soil and aquatic environments. We have evaluated the influence of pH and di valent cation levels on the growth of M. tuberculosis and seven other mycob acterial species, In a defined medium, the growth of M. tuberculosis was ve ry restricted by acidic pH. Higher levels of Mg2+ were required for growth of M, tuberculosis in mildly acidic media (pH 6.0 to 6.5) compared to pH 7. 0 medium. The divalent cations Ca2+, Zn2+, or Mn2+ could not replace Mg2+ d uring growth at pH 6.25, but Ca2+ could at least partially substitute for M g2+ during growth at pH 7.0. Among eight species of mycobacteria tested, th ere was a diversity of growth rates in media with acidic pH and low Mg2+ le vels. M. tuberculosis was the most restricted in growth at pH 6.0, and all of this growth required elevated levels of Mg2+. M. kansasii and M, smegmat is also grew very poorly in acidic media with limiting Mg2+. M. fortuitum, M. marinum, M. scrofulaceum, M. avium, and M. chelonae grew at pH 6.0 in an unrestricted manner. These results demonstrate that M. tuberculosis is uni que among the mycobacteria in its extreme sensitivity to acid and indicate that M. tuberculosis must acquire sufficient Mg2+ in order to grow in a mil dly acidic environment such as within the phagosome of macrophages.