M. Kubo-irie et al., Spermiogenesis in the stag beetle, Aegus lavicollis Waterhouse (Coleoptera, Lucanidae), with special reference to the centriole adjunct, INVERTEBR R, 37(3), 2000, pp. 223-231
Ultrastructural changes during spermiogenesis in the stag beetle, Aegus lav
icollis, were studied with special attention to the organizing process of t
he centriole adjunct. In early spermatids the formation of acrosome and fla
gellum occurs simultaneously in the cytoplasm before nuclear condensation.
The centriole adjunct first appears as a sheath surrounding a centriole in
a concavity of the nucleus from which the flagellar axoneme is organized. A
mass of electron-dense material accumulates progressively in the vicinity
of the centriole. As nuclear transformation proceeds, electron-dense materi
al is found at the posterior end of the nucleus and extends beyond the cent
riolar region to form a partial sheath around mitochondrial derivatives. El
ectron-dense material seems to contribute to the formation of the centriole
adjunct because the former is closely attached to the latter until the lat
ter is completely formed. In mature spermatozoa the centriole adjunct is ch
aracterized by its peculiar disposition, extending across about half the le
ngth of the flagellum and partly covering the outside of two mitochondrial
derivatives.