Molecular mechanism of calcium channel block by isradipine - Role of a drug-induced inactivated channel conformation

The role of the inactivated channel conformation in the molecular mechanism of Ca2+ channel block by the 1,4-dihydropyridine (DHP) (+)-isradipine was analyzed in L-type channel constructs (alpha(1LC); Berjukow, S., Gapp, F., Aczel, S., Sinnegger, M. J., Mitterdorfer, J., Glossmann, H., and Hering, S . (1999) J. Biol. Chem. 274, 6154-6160) and a DHP-sensitive class A Ca2+ ch annel mutant (alpha(1A-DHP); Sinnegger, M. J,, Wang, Z,, Grabner, RI., Heri ng, S., Striessnig, J., Glossmann, H., and Mitterdorfer, J. (1997) J. Biol Chem. 272, 27686-27693) carrying the high affinity determinants of the DHP receptor site but inactivating at different rates. Ca2+ channel inactivatio n was modulated by coexpressing the alpha(1A-DHP)- or alpha(1LC)-subunits i n Xenopus oocytes with either the beta(2a)- or the beta(1a)-subunit and ami no acid substitutions in L-type segment IVS6 (I1497A, I1498A, and V1504A). Contrary to a modulated receptor mechanism assuming high affinity DHP bindi ng to the inactivated state we observed no clear correlation between steady state inactivation and Ca2+ channel block by (+)-isradipine: (i) a 3-fold larger fraction of alpha(1A-DHP/beta 1a), channels in steady state inactiva tion at -80 mV (compared with alpha(1A-DHP)/beta(2a)) did not enhance the b lock by (+)-isradipine; (ii) different steady state inactivation of alpha(1 Lc) mutants at -30 mV did not correlate with voltage-dependent channel bloc k; and (iii) the midpoint-voltages of the inactivation curves of slowly ina ctivating L-type constructs and more rapidly inactivating alpha(1Lc)/beta(1 a) channels were shifted to a comparable extent to more hyperpolarized volt ages. A kinetic analysis of (+)-isradipine interaction with different L-typ e channel constructs revealed a drug-induced inactivated state. Entry and r ecovery from drug-induced inactivation are modulated by intrinsic inactivat ion determinants, suggesting a synergism between intrinsic inactivation and DHP block.