Ultraviolet light-induced stimulation of the JNK mitogen-activated proteinkinase in the absence of Src family tyrosine kinase activation

Citation
K. Amdjadi et Bm. Sefton, Ultraviolet light-induced stimulation of the JNK mitogen-activated proteinkinase in the absence of Src family tyrosine kinase activation, J BIOL CHEM, 275(29), 2000, pp. 22520-22525
Citations number
59
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
275
Issue
29
Year of publication
2000
Pages
22520 - 22525
Database
ISI
SICI code
0021-9258(20000721)275:29<22520:ULSOTJ>2.0.ZU;2-7
Abstract
In T cells, the JNK mitogen-activated protein kinase is activated by simult aneous stimulation of the T-cell receptor and CD28 or by a number of stress stimuli including ultraviolet light, hydrogen peroxide, and anisomycin, Lc k, a Src family kinase, is essential for T-cell receptor-mediated activatio n of JNK, We asked whether Lck was also involved in stress-mediated activat ion of JNK, JNK was activated by ultraviolet light irradiation in all of th e four T-cell lines we examined, but Lck was not. Additionally, JNK activat ion by ultraviolet light, hydrogen peroxide, and anisomycin was completely normal in T cells lacking Lck, These data suggest that Lck is not activated by ultraviolet light irradiation, nor is it required for JNK activation in T cells by any of the stress stimuli we tested. We also examined JNK activ ation by ultraviolet light in mouse fibroblasts expressing no known Src kin ases, The activation of JNK by ultraviolet light was completely normal in t hese cells. Finally, treatment of lymphoid and epithelial cells with a Src kinase family inhibitor PPB-reduced tyrosine phosphorylation of cellular pr oteins markedly without affecting ultraviolet light-induced activation of J NK, These results suggest that Src kinases are not essential for ultraviole t light-induced activation of JNK in a diverse variety of cell types.