Effects of 10 days of endurance exercise training on the suppression of whole body and regional lipolysis by insulin

The aim of this study was to evaluate in premenopausal women (10 sedentary obese women) the effects of 10 days of exercise on the suppression of whole body and regional lipolysis by insulin. Lipolysis was determined using H-2 (5)-glycerol infusion and microdialysis of sc adipose tissue during a two-s tage hyperinsulinemic-euglycemic clamp [10 (LO) and 20 (MO) mU/m.min]. Micr odialysis probes were positioned in abdominal and femoral sc adipose tissue to monitor interstitial glycerol and Mood flow. Basal plasma glycerol was 86.7 +/- 17.0 and 100.3 +/- 19.8 mu mol/L before and after training, respec tively (P < 0.05), Plasma glycerol was suppressed to a greater extent after [to 47 +/- 5% (LO) and 42 +/- 5% (MO) of basal] than before [to 62 +/- 8% (LO) and 55 +/- 8% (MO) of basal] training. The rate of appearance of glyce rol was suppressed to 49 +/- 18 and 40 +/- 5% of basal during LO and to 38 +/- 5% and 30 +/- 4% of basal during MO (P < 0.05) before and after trainin g, respectively. There were no differences in the suppression of lipolysis in abdominal as well as femoral sc adipose tissue as evidenced by similar r eductions in dialysate glycerol levels before and after training in each of these tissues. The results indicate that the antilipolytic response to ins ulin can be improved through endurance exercise training. Intraabdominal ad ipose tissue or skeletal muscle may he the site of improved antilipolytic r esponse to insulin after training, as improvement was not evident in abdomi nal or femoral sc adipose tissue.