Multimedia tools for learning fluorescence: A spectrofluorometer simulator

A new teaching method, using multimedia software, is in progress at our uni versity. Running on a PC computer, it allows self-formation in molecular sp ectroscopy. A new software package devoted to photoluminescence (ISPL) is c urrently in progress. The concepts are introduced by using numerous graphs, animations, and simulations. The software allows electronic book facilitie s (search function, glossary, and bookmarks, for instance) and self-assessm ent. Tedious and time-consuming training is always necessary to acquire exp erimental skill. This is particularly true in the case of luminescence beca use numerous artifacts are possible and the number of parameters to be opti mized is very large. In this view, a spectrofluorometer simulator appears t o be a valuable tool that makes this training easy and fast: after choosing a set of experimental parameters, the computer displays immediately the co rresponding spectrum. The influence of every experimental parameter is simu lated: monochromator slit width, excitation or emission wavelengths, xenon source type, high-voltage photomultiplier tube, etc. The nature of solvents , with first- and second-order Rayleigh and Raman scattering, is managed. S ample geometry and concentration, with a corresponding inner filter, are al so simulated. Obviously, there is no risk of damaging the virtual photomult iplier tube.