Nuclear magnetic resonance spectroscopy and reversed-phase high-performance liquid chromatography of peracetylated digalactosyldiacylglycerols

A simple method for the peracetylation of digalactosyldiacylglycerols is de scribed. The peracetylated compounds were isolated by thin-layer chromatogr aphy and subjected to nuclear magnetic resonance spectroscopy for structure elucidation. The proton signals at acetylated sites are shifted downfield compared to the nonderivatized compounds, resulting in improved resolution. The peracetylated compounds were also subjected to molecular species analy sis by reversed-phase high-performance liquid chromatography. The chromatog raphy behavior of the peracetylated compounds was similar to that of triacy lglycerols, displaying narrow and symmetrical peaks yielding a highly resol ved molecular species profile.