Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors

Authors
Seppen, J Barry, SC Klinkspoor, JH Katen, LJ Lee, SP Garcia, JV Osborne, WRA
Citation
J. Seppen et al., Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors, J VIROLOGY, 74(16), 2000, pp. 7642-7645
Citations number
18
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
74
Issue
16
Year of publication
2000
Pages
7642 - 7645
Database
ISI
SICI code
0022-538X(200008)74:16<7642:AGTIQH>2.0.ZU;2-L
Abstract
Intestinal epithelial cells secrete a protective luminal mucus barrier inhi biting viral gene transfer. Quiescent, polarized monolayers of primary epit helial cells from dog gallbladder and human colon are efficiently transduce d through the apical mucus side by lentivirus vectors, suggesting their app lication to intestinal gene therapy.