Thin-layer chromatographic detection of denatonium saccharide (Amerex) in denatured alcohol

Authors
Rathod, DS Mali, BD Garad, MV Patil, VA
Citation
Ds. Rathod et al., Thin-layer chromatographic detection of denatonium saccharide (Amerex) in denatured alcohol, J PL CHROM, 13(2), 2000, pp. 146-148
Citations number
16
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
ISSN journal
09334173 → ACNP
Volume
13
Issue
2
Year of publication
2000
Pages
146 - 148
Database
ISI
SICI code
0933-4173(200003/04)13:2<146:TCDODS>2.0.ZU;2-P
Abstract
A thin layer chromatographic method is described for detection of the bitte rant denatonium saccharide (Amerex) in denatured spirit. On chlorination an d treatment with o-tolidine the bitterant yields an intense blue spot. Othe r denaturants such as pyridine bases and caoutchoucine generally present in denatured spirit do not give a positive reaction with this reagent and hen ce do not interfere with the test. The detection limit for denatonium sacch aride is 0.2 mu g (10 mu g mL(-1)) per spot.