Influence of the surface pressure on the organization of mixed Langmuir-Blodgett films of octadecylamine and butyrylcholinesterase. 1. Film preparation at the air-water interface

In order to obtain good biosensor characteristics, an investigation on the mixed octadecylamine/ butyrylcholinesterase monolayer at the air-water inte rface is conducted. The kinetics of the adsorption of the enzyme at the air -water interface is dependent on time, concentration, and the ionic interac tion with the amphiphilic polar groups. From the surface pressure-molecular area isotherms and their stabilities, a model of the mixed film at the air -water interface is proposed and experimental conditions leading to a stabl e film are defined and explained.