Isolation and characterization of transposon-induced chlorate resistant mutants of the cyanobacterium Anabaena species PCC 7120

Authors
Rai, AK Tiwari, SP
Citation
Ak. Rai et Sp. Tiwari, Isolation and characterization of transposon-induced chlorate resistant mutants of the cyanobacterium Anabaena species PCC 7120, MICROBIOS, 102(402), 2000, pp. 103-112
Citations number
18
Categorie Soggetti
Microbiology
Journal title
MICROBIOS
ISSN journal
00262633 → ACNP
Volume
102
Issue
402
Year of publication
2000
Pages
103 - 112
Database
ISI
SICI code
0026-2633(2000)102:402<103:IACOTC>2.0.ZU;2-T
Abstract
Mutants of Anabaena so. PCC 7120 resistant to chlorate were isolated using transposon mutagenesis. The Anabaena population of 5 x 10(7) cells ml(-1) a nd log phase Escherichia coli cultures in undisturbed conditions produced m aximum exconjugants. Nitrate-promoted growth and cellular constituents obse rved in the parent were absent in the mutants. Nitrate repressed heterocyst formation and N-2-fixation in the parent, but had little or no effect on t he mutants.