Binding specificity of monoclonal antibody AD2: influence of the phosphorylation state of tau

Authors
Torreilles, F Roquet, F Granier, C Pau, B Mourton-Gilles, C
Citation
F. Torreilles et al., Binding specificity of monoclonal antibody AD2: influence of the phosphorylation state of tau, MOL BRAIN R, 78(1-2), 2000, pp. 181-185
Citations number
17
Categorie Soggetti
Neurosciences & Behavoir
Journal title
MOLECULAR BRAIN RESEARCH
ISSN journal
0169328X → ACNP
Volume
78
Issue
1-2
Year of publication
2000
Pages
181 - 185
Database
ISI
SICI code
0169-328X(20000531)78:1-2<181:BSOMAA>2.0.ZU;2-S
Abstract
Using recombinant human tau protein phosphorylated by a brain extract and t he glycogen synthase kinase-3 beta in the absence or the presence of hepari n, we showed that phosphorylation-dependent antibody AD2 recognition only r equires phosphorylated Ser-396. By the Spot multiple peptide synthesis meth od, we showed that Tyr-394, Ser(P)-396 and Pro-397 are critical for AD2 bin ding. A decrease in the binding of AD2 was observed with increasing phospho rylation of residues in the vicinity of SerP-396. (C) 2000 Published by Els evier Science B.V. All rights reserved.