Evaluation of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry for the identification and quantification of desulphoglucosinolates

The use of liquid chromatography-atmospheric pressure chemical ionisation-m ass spectrometry has been investigated as a potential method for both the i dentification and quantification of desulphoglucosinolates. The results ind icate that this methodology is particularly useful as a rapid technique for the confirmation of the identity of desulphoglucosinolates and has, in ful l scan mode, limits of detection at least an order of magnitude lower than is normally achieved by conventional high pressure liquid chromatography wi th UV detection. It has been demonstrated that, using a per-deuterated desu lphoglucosinolate as an internal standard, quantification could be achieved , but for accurate analysis at low concentrations it would be necessary to produce independent calibration curves for each desulphoglucosinolate prese nt in the sample. Copyright (C) 2000 John Wiley & Sons, Ltd.