Comparison of extraction and clean-up methods for the analysis of friedelan-3-ol and friedelin from leaves of Maytenus aquifolium Martius (Celastraceae)

A comparative study of three extraction methods (maceration/sonication, Sox hlet and supercritical fluid extraction) and three clean-up procedures for chromatographic analysis (high resolution gas chromatography with flame ion isation detection; HRGC-FID) of the triterpenes friedelan-3-ol and friedeli n from leaves of Maytenus aquifolium Martins (Celastraceae), one of the spe cies known in Brazil as "espinheira santa", has been conducted. Supercritic al fluid extraction utilising carbon dioxide modified with ethanol was show n to be the most effective extraction method, while clean-up utilising Flor isil((R)) as adsorbent was the most appropriate for further HRGC-FID quanti tative analysis of the extracts; Copyright (C) 2000 John Wiley & Sons, Ltd.