One step more towards taxane production through enhanced Taxus propagation

Citation
Jp. Majada et al., One step more towards taxane production through enhanced Taxus propagation, PL CELL REP, 19(8), 2000, pp. 825-830
Citations number
18
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
07217714 → ACNP
Volume
19
Issue
8
Year of publication
2000
Pages
825 - 830
Database
ISI
SICI code
0721-7714(200007)19:8<825:OSMTTP>2.0.ZU;2-S
Abstract
We have developed a high-yielding procedure for the in vitro propagation of juvenile material of Taxus baccata involving a combination of seed handlin g and culture on WP culture medium supplemented with sucrose (2%), activate d charcoal (0.5%) and BAP (22.19 mM) for 30 days, followed by 40 days on ho rmone-free medium. Shoot apical ends should be decapitated to obtain propag ation rates up to 12- to 18-fold per subculture period (70 days). In this w ay the high genetic variability of the juvenile material can be used in the most productive way. In addition to producing large numbers of yew plants (difficult to get by traditional methods), this procedure allows the fast s creening of individuals for their taxane content. A negative correlation be tween growth and secondary metabolite content was found for paclitaxel. The positive correlation with 10-deacetyl baccatin III accumulation reflects o nce more the commercial viability of using 10-deacetyl baccatin III extract ion as an alternative to taxane production, but this time opening up the po ssibility of selecting genotypes with both characteristics: fast growth and high productivity.