A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin

Authors
Huang, Y Mechref, Y Tian, J Gong, H Lennarz, WJ Novotny, MV
Citation
Y. Huang et al., A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin, RAP C MASS, 14(14), 2000, pp. 1233-1237
Citations number
25
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
09514198 → ACNP
Volume
14
Issue
14
Year of publication
2000
Pages
1233 - 1237
Database
ISI
SICI code
0951-4198(2000)14:14<1233:ASSPFE>2.0.ZU;2-K
Abstract
A simple microadsorption technique is described to remove detergent additiv es from oligosaccharide samples before their mass spectrometric analysis, T he described methodology has been validated with submicrogram quantities of contaminated glycoproteins. This procedure is applicable to investigating minute quantities of glycans in both the positive- and negative-ion mode of matrix-assisted laser desorption/ionization mass spectrometry. Copyright ( C) 2000 John Wiley & Sons, Ltd.