Bioassays using caged fish, bivalves, and other macroinvertebrates (in situ
toxicity tests) supplement traditional eco-toxicological approaches such a
s laboratory toxicity tests and evaluation of benthic invertebrate or fish
communities. In situ toxicity tests incorporate complex site-specific condi
tions (e.g., suspended solids, light, dissolved oxygen, pH, salinity, and t
emperature), which may alter contaminant toxicity and/or bioavailablity. Fi
sh and bivalves have been used most frequently due to technology transfer f
rom aquaculture methodologies and other concerns about these animal groups.
More recently, laboratory methods for testing commonly used macroinvertebr
ates (e.g., cladocerans, amphipods, and midges) have been developed for use
in the field. A concise summary of the uses and applications of in situ te
sting (e.g., stressor identification, chronic bioaccumulation studies, stor
mwater runoff assessment, evaluation of photoinduced toxicity at PAH-contam
inated sites, and assessment of episodic acidification of streams) is provi
ded.