The design and synthesis of a 5-HETE affinity chromatography ligand for 5-hydroxyeicosanoid dehydrogenase

Authors
Khanapure, SP Saha, G Powell, WS Rokach, J
Citation
Sp. Khanapure et al., The design and synthesis of a 5-HETE affinity chromatography ligand for 5-hydroxyeicosanoid dehydrogenase, TETRAHEDR L, 41(31), 2000, pp. 5807-5811
Citations number
14
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
41
Issue
31
Year of publication
2000
Pages
5807 - 5811
Database
ISI
SICI code
0040-4039(20000729)41:31<5807:TDASOA>2.0.ZU;2-8
Abstract
The first total synthesis of an omega-amino 5-HETE derivative 27 has been a ccomplished by a new counterclockwise strategy, in which C-1 is constructed first and C-20 last. The omega-amino 5-HETE derivative was transformed to an affinity chromatography ligand, the biotinylated 5-HETE 30. This affinit y chromatography ligand is aimed at purifying the 5-hydroxyeicosanoid dehyd rogenase enzyme, which is responsible for the conversion of 5-HETE to 5-oxo -ETE, a potent eosinophil chemotactic factor. (C) 2000 Elsevier Science Ltd . All rights reserved.