Possible involvement of bcl-2 suppression in wild-type p53 gene-dependent cell growth repression in rat osteosarcoma cells

Citation
K. Honoki et al., Possible involvement of bcl-2 suppression in wild-type p53 gene-dependent cell growth repression in rat osteosarcoma cells, TOX PATHOL, 28(4), 2000, pp. 575-579
Citations number
32
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICOLOGIC PATHOLOGY
ISSN journal
01926233 → ACNP
Volume
28
Issue
4
Year of publication
2000
Pages
575 - 579
Database
ISI
SICI code
0192-6233(200007/08)28:4<575:PIOBSI>2.0.ZU;2-6
Abstract
We recently obtained 3 cloned cell lines demonstrating the p53 mutation fro m a lung metastatic nodule of a rat transplantable osteosarcoma. In this st udy, we applied wild-type p53 gene transfer to the rat osteosarcoma cells b y lipofection to investigate the effects on cell growth, expression of gene s such as waf1/p21, bcl-2, and bax, and nucleosomal DNA fragmentation due t o apoptosis. Reconstitution of the p53 gene inhibits cellular growth, and t his growth-suppressive effect is partly due to apoptosis involving bcl-2 ge ne suppression in this tumor type. This rat osteosarcoma model is similar i n biologic behavior to human cases and thus is very suitable for further in vestigation of tumorigenesis and gene therapy for osteosarcoma.