The common weed Macroptilium lathyroides is not a source of crop-infectinggeminiviruses from Jamaica

The molecular characterization of bipartite geminiviruses infecting the com mon leguminous weed Macroptilium lathyroides was accomplished using polymer ase chain reaction (PCR), deoxyribonucleic acid (DNA) hybridization, and nu cleotide sequence analysis. The predicted PCR-amplified fragments for DNA-A and DNA-B showed that M. lathyroides was infected with bipartite geminivir uses with a genome organization similar to other Western Hemisphere geminiv iruses. Nucleotide sequence analysis showed that M. lathyroides from Jamaic a was infected with two different viruses. Phylogenetic analysis of these m acroptilium viruses from Jamaica and Western Hemisphere viruses, including three Viruses from Jamaica indicated that macroptilium golden mosaic virus Jamaica strain 1 (MacGMV-JM1) was phylogenetically related to PYMV and SiGM V-JM, while the other virus, macroptilium golden mosaic virus Jamaica strai n 2 (MacGMV-JM2) was closely related to two Jamaican viruses, TDLCV and WGM V. The results suggest that crop and weed geminiviruses from Jamaica are re lated but distinct, and that M. lathyroides is host to previously described crop-infecting geminiviruses from Jamaica.