Rapid and reliable protein extraction from yeast

The methods currently used for protein extraction from yeast are either lab orious or insufficiently reliable. Here I report a method for protein extra ction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for dif ferent strains of Saccharomyces ceverisiae and for yeast Hansenula polymorp ha DL-I. It yields virtually complete extraction independently of the strai n, growth conditions and protein molecular weight and allows working with s mall amounts of yeast cells grown on agar plates. Copyright (C) 2000 John W iley & Sons, Ltd.