Cloning and sequence analysis of the Candida boidinii ADE2 gene

Candida boidinii ADE2 gene (phosphoribosyl-5-aminoimidazole carboxylase; AI RC, EC , 1. 1. 21) has been cloned by homology to the Saccharomyces cerevis iae ADE2 gene. The cloned C, boidinii ADE2 gene complemented the ade2 mutat ion of S, cerevisiae. Sequence analysis showed a single open reading frame of 1719 nucleotides coding for a polypeptide of 573 residues, Comparison of the deduced amino acid sequence with those of AIRC enzymes from other yeas ts-shelved marked homology among yeast AIRCs, The sequence has already been submitted to DDBJ/EBML/GenBank under Accession No. AB034950. Copyright (C) 2000 John Whey & Sons, Ltd.