Functional cloning of the adenylate cyclase gene of Candida albicans in Saccharomyces cerevisiae within a genomic fragment containing five other genes, including homologues of CHS6 and SAP185

We have cloned a genomic fragment of Candida albicans by complementation of a Saccharomyces cerevisiae cyr1 mutant. This fragment contains the two-thi rds C-terminal part of the adenylate cyclase CaCYR1. The complete gene has been sequenced from PCR fragments amplified from genomic DNA, and contains an ORF of 1690 amino acids closely related to other fungal adenylate cyclas es. Adjacent to the adenylate cyclase gene, we have sequenced six other put ative genes. CaCHS6, CaYNL191 and CaYJL098 are named on the basis of their close similarity with S. cerevisiae genes. ORFs CaYJL097a and CaYJL097b rep resent two repeated homologues of the S. cerevisiae YJL09w, which probably arose from an ancient duplication. The last one is a hypothetical ORF, CaYK R049, which presents only a very weak similarity with YKR049. The S. cerevi siae homologues of three of these genes are co-localized on chromosome X bu t with a different order and orientation. The EMBL Accession No. of CaCYR1 is AJ250877 and the insert containing the other genes is No. AJ250310. Copy right (C) 2000 John Whey & Sons, Ltd.