Functional cloning of the adenylate cyclase gene of Candida albicans in Saccharomyces cerevisiae within a genomic fragment containing five other genes, including homologues of CHS6 and SAP185
L. Mallet et al., Functional cloning of the adenylate cyclase gene of Candida albicans in Saccharomyces cerevisiae within a genomic fragment containing five other genes, including homologues of CHS6 and SAP185, YEAST, 16(10), 2000, pp. 959-966
We have cloned a genomic fragment of Candida albicans by complementation of
a Saccharomyces cerevisiae cyr1 mutant. This fragment contains the two-thi
rds C-terminal part of the adenylate cyclase CaCYR1. The complete gene has
been sequenced from PCR fragments amplified from genomic DNA, and contains
an ORF of 1690 amino acids closely related to other fungal adenylate cyclas
es. Adjacent to the adenylate cyclase gene, we have sequenced six other put
ative genes. CaCHS6, CaYNL191 and CaYJL098 are named on the basis of their
close similarity with S. cerevisiae genes. ORFs CaYJL097a and CaYJL097b rep
resent two repeated homologues of the S. cerevisiae YJL09w, which probably
arose from an ancient duplication. The last one is a hypothetical ORF, CaYK
R049, which presents only a very weak similarity with YKR049. The S. cerevi
siae homologues of three of these genes are co-localized on chromosome X bu
t with a different order and orientation. The EMBL Accession No. of CaCYR1
is AJ250877 and the insert containing the other genes is No. AJ250310. Copy
right (C) 2000 John Whey & Sons, Ltd.