Effect of immersion fluid temperature on the chicken embryo in teratogenicity tests: Short communication

The influence of immersion fluid temperature on the development of the chic ken embryo was studied on the day most commonly used for treating incubated eggs in teratological trials. Embryonated eggs were immersed in tap water for 30 min on the 12th day of incubation at 22-25 degrees C or at incubatio n temperature without a waiting time or after 30 min. The incubation was th en continued and the eggs were processed on day 19 of the incubation period . Treatment of eggs at 22-25 degrees C caused a significant increase in emb ryonic mortality, while the 30-min waiting time did not exert an influence on embryogenesis.