Al. Simanov et al., ANALYSIS OF PRODUCT COMPOSITION OF POLYMERIZATION REACTION CATALYZED BY POLYNUCLEOTIDE PHOSPHORYLASE FROM THERMUS-THERMOPHILUS, Biochemistry, 59(5), 1994, pp. 537-540
The polymerization of ADP catalyzed by polynucleotide phosphorylase wa
s investigated. Analysis of reaction mixture by high performance ion-e
xchange liquid chromatography near the beginning of the reaction revea
led oligonucleotides containing no more than five monomer residues. In
creasing the NaCl concentration to 1 M induced a lag phase in the reac
tion and induced the accumulation of oligomers. Simultaneously increas
ing the NaCl and magnesium ion concentrations resulted in the disappea
rance of the lag phase and in the increase in the velocity of polynucl
eotide synthesis which was slowed at high ionic strength.