Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Authors
Fukuda, N Tanaka, H Shoyama, Y
Citation
N. Fukuda et al., Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines, ANALYST, 125(8), 2000, pp. 1425-1429
Citations number
37
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
00032654 → ACNP
Volume
125
Issue
8
Year of publication
2000
Pages
1425 - 1429
Database
ISI
SICI code
0003-2654(2000)125:8<1425:AOEWBA>2.0.ZU;2-K
Abstract
A combination of ELISA, Western blotting and immunoaffinity concentration u sing an anti-ginsenoside Rb1 monoclonal antibody was applied for qualitativ e and quantitative surveys of ginsenoside Rb1 and related ginsenosides in r oots and traditional Chinese herbal medicines. To improve the low correlati on between ELISA and HPLC analysis, the crude extract of roots was immunoaf finity concentrated to make evident the effect of malonyl ginsenoside Rb1. Immunoaffinity column chromatography also concentrated an unknown ginsenosi de which had the same cross-reaction with ginsenoside Rb1.