Nitrosomethylurea induces nuclear and cytoplasmic chlorophyll mutations inNicotiana glauca Grahm

Citation
M. Marcotrigiano et G. Hackett, Nitrosomethylurea induces nuclear and cytoplasmic chlorophyll mutations inNicotiana glauca Grahm, ANN BOTANY, 86(2), 2000, pp. 293-298
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
ANNALS OF BOTANY
ISSN journal
03057364 → ACNP
Volume
86
Issue
2
Year of publication
2000
Pages
293 - 298
Database
ISI
SICI code
0305-7364(200008)86:2<293:NINACC>2.0.ZU;2-N
Abstract
Pigment markers, critical for developmental analysis, were not previously a vailable in Nicotiana glauca Grahm. Imbibed seed soaked in 1 mM solution of the mutagen nitrosomethylurea (NMU) yielded variegated variants whereas wa ter-soaked seed gave rise to no variants. Two of the variants were characte rized via progeny analysis and transmission electron microscopy. Both M-1 v ariants were male sterile but female fertile. Variant 97-105 was a yellow-g reen seedling possessing somewhat normal plastids and displaying characteri stics of a codominant nuclear mutation. When variant 97-105 females were cr ossed with wild-type males, restored male fertility was evident in some see dlings. Variant 97-411 was variegated with green, light green and white lea f regions. Whiter regions displayed highly aberrant plastids. Eventually, p lastid sorting-out in 97-411 resulted in a periclinal chloroplast chimera. Progeny of heteroplastidic shoots of 97-411 segregated to variegated, green , or white seedlings when 97-411 was used as the maternal parent. NMU was e ffective in obtaining both nuclear and cytoplasmic pigment mutants in a spe cies where none have been previously reported. (C) 2000 Annals of Botany Co mpany.