We developed a method to purify appressoria of the bean anthracnose fungus
Colletotrichum lindemuthianum for biochemical analysis of the cell surface
and to compare appressoria with other fungal structures. We used immunomagn
etic separation after incubation of infected bean leaf homogenates with a m
onoclonal antibody that binds strongly to the appressoria. Preparations wit
h a purity of >90% could be obtained. Examination of the purified appressor
ia by transmission electron microscopy showed that most had lost their cyto
plasm. However, the plasma membrane was retained, suggesting that there is
some form of attachment of this membrane to the cell wall, The purified app
ressoria can be used for studies of their cell surface, and we have shown t
hat there are clear differences in the glycoprotein constituents of cell wa
lls of appressoria compared with mycelium.