Mycobacteria were isolated from defrost water and tissue of sole (Solea sol
ea), hake (Merluccius merluccius), cod (Gadus morhua), ling (Genypterus bla
codes), and monkfish (Lophius piscatorius) on Lowenstein-Jensen medium afte
r incubation at different temperatures. Samples of frozen fish were obtaine
d under sterile conditions inside a refrigeration chamber (-18 to -22 degre
es C) in a wholesale market from which these products are distributed to sh
ops for retail sale and human consumption.