M. Sanchez-contreras et al., PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti, APPL ENVIR, 66(8), 2000, pp. 3621-3623
A PCR identification method in which four primers that recognize homologous
conserved regions in the Sinorhizobium meliloti genome are used was develo
ped and tested. The regions used for identification were the nodbox 4 locus
, which is located in one of the symbiotic megaplasmids,and the mucR gene,
which is located in the chromosome. The new method was used to establish a
collection of S. meliloti strains from polluted soils.