Quantitative determination of the biodegradable polymer poly(beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics

Fourier transform infrared (FTIR) spectroscopy in combination with the part ial least squares (PLS) multivariative statistical technique was used for q uantitative analysis of the poly(P-hydroxybutyrate) (PHB) contents of bacte rial cells. A total of 237 replicate spectra from 34 samples were obtained together with gas chromatography-determined reference PHB contents. Using t he PLS regression, we were able to relate the infrared spectra to the refer ence PHB contents, and the correlation coefficient between the measured and predicted values for the optimal model with a standard error of prediction of 1.49% PHB was 0.988. With this technique, there are no solvent requirem ents, sample preparation is minimal and simple, and analysis time is greatl y reduced; our results demonstrate the potential of FTIR spectroscopy as an alternative to the conventional methods used for analysis of PHB in bacter ial cells.