A. Hashimoto et T. Kameoka, Mid-infrared spectroscopic determination of sugar contents in plant-cell culture media using an ATR method, APPL SPECTR, 54(7), 2000, pp. 1005-1011
A simple, rapid, and accurate method for determining sugar contents in plan
t-cell culture media by mid-infrared (MIR) spectroscopy was developed by us
ing a Fourier transform infrared (FT-IR) spectrometer equipped with an atte
nuated total reflectance (ATR) accessory. We examined the potential of this
method by comparison with a high-performance liquid chromatography (HPLC)
method and applied it to the determination of sugar consumption by plant ce
lls. The cultivation was carried out with Nicotiana tabacum cv. Bright Yell
ow No. 2 cells. We determined that MIR spectroscopy is at least as useful a
s the HPLC method for the sugar content determination in the culture medium
and may be available to monitor the overall cell metabolisms. This study r
epresents an important step in the development of nonintensive on-line moni
toring devices for bioreactors and of simple, rapid, and accurate analysis
of the cellular metabolism through the composition changes in media.