The bystander effect in photodynamic inactivation of cells

Citation
J. Dahle et al., The bystander effect in photodynamic inactivation of cells, BBA-GEN SUB, 1475(3), 2000, pp. 273-280
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN journal
03044165 → ACNP
Volume
1475
Issue
3
Year of publication
2000
Pages
273 - 280
Database
ISI
SICI code
0304-4165(20000726)1475:3<273:TBEIPI>2.0.ZU;2-R
Abstract
Treatment of MDCK II cells with the lipophilic photosensitizer tetra(3-hydr oxyphenyl)porphyrin and light was found to induce a rapid apoptotic respons e in a large fraction of the cells. Furthermore, the distribution of apopto tic cells in microcolonies of eight cells was found to be different from th e binomial distribution, indicating that the cells are not inactivated inde pendently, but that a bystander effect is involved in cell killing by photo dynamic treatment, The observation of a bystander effect disagrees with the common view that cells are inactivated only by direct damage and indicates that communication between cells in a colony plays a role in photosensitiz ed induction of apoptosis. The degree of bystander effect was higher for ce lls dying by necrosis than for cell dying by apoptosis. (C) 2000 Elsevier S cience B.V. All rights reserved.