Crystallization and preliminary X-ray crystallographic analysis of NADPH: azodicarbonyl/quinone oxidoreductase, a plant zeta-crystallin

Arabidopsis thaliana P1 protein was crystallized using the hanging drop vap or-diffusion method in 0.1 M piperazine-1,4-bis(2-ethanesulfonic acid) buff er, containing 14% polyethylene glycol 6000 and 0.2 M magnesium acetate at pH 6.5 and 20 degrees C. The crystals are orthorhombic and belong to the sp ate group P2(1)2(1)2(1) with unit cell dimensions of a = 49.8, b = 122.4 an d c = 139.9 Angstrom. The diffraction data up to 2.9 Angstrom were collecte d by a multiwire area detector. (C) 2000 Elsevier Science B.V. All rights r eserved.