The solid phase coupling of peptide segments catalyzed by the subtilisin-sodium dodecyl sulfate complex

Authors
Kolobanova, SV Filippova, IY Lysogorskaya, EN Getun, IV Bacheva, AV Oksenoit, ES Stepanov, VM
Citation
Sv. Kolobanova et al., The solid phase coupling of peptide segments catalyzed by the subtilisin-sodium dodecyl sulfate complex, BIOORG KHIM, 26(6), 2000, pp. 411-416
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
26
Issue
6
Year of publication
2000
Pages
411 - 416
Database
ISI
SICI code
0132-3423(200006)26:6<411:TSPCOP>2.0.ZU;2-8
Abstract
The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the cou pling of peptide segments on a solid phase in organic medium. By a two-stag e enzymic condensation of peptide fragments on aminosilochrom (A) containin g Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala -Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It w as shown that the condensation products can be split off from the support u sing the Met residue cleavage by BrCN.