Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC

Authors
Shida, T Hattori, H Ise, F Sekiguchi, J
Citation
T. Shida et al., Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC, BIOS BIOT B, 64(7), 2000, pp. 1522-1525
Citations number
13
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
7
Year of publication
2000
Pages
1522 - 1525
Database
ISI
SICI code
0916-8451(200007)64:7<1522:OPACOB>2.0.ZU;2-6
Abstract
N-acetylmuramoyl-L-alanine amidase CwIC of Bacillus subtilis was overproduc ed in Escherichia coil and purified 21-fold. The amidase hydrolyzed type A cell walls such as B. subtilis. The amidase bound slightly to the Microbact erium lacticum cell wall (type B), but did not entirely hydrolyze it. The p resence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation.