T. Shida et al., Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC, BIOS BIOT B, 64(7), 2000, pp. 1522-1525
N-acetylmuramoyl-L-alanine amidase CwIC of Bacillus subtilis was overproduc
ed in Escherichia coil and purified 21-fold. The amidase hydrolyzed type A
cell walls such as B. subtilis. The amidase bound slightly to the Microbact
erium lacticum cell wall (type B), but did not entirely hydrolyze it. The p
resence of calcium or magnesium ion increased the resistance of the amidase
to heat denaturation.