Simultaneous detection of different glycosidase activities by F-19 NMR spectroscopy

Authors
Albert, M Repetschnigg, W Ortner, J Gomes, J Paul, BJ Illaszewicz, C Weber, H Steiner, W Dax, K
Citation
M. Albert et al., Simultaneous detection of different glycosidase activities by F-19 NMR spectroscopy, CARBOHY RES, 327(4), 2000, pp. 395-400
Citations number
18
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
327
Issue
4
Year of publication
2000
Pages
395 - 400
Database
ISI
SICI code
0008-6215(20000807)327:4<395:SDODGA>2.0.ZU;2-1
Abstract
A fast method for the simultaneous detection of different glycosidolytic ac tivities in commercially available enzyme preparations and crude culture fi ltrates was found in using, as substrate, a mixture of different glycosyl f luorides and F-19 NMR spectroscopy as a screening technique. Accompanying s tudies regarding the hydrolytic stability of these fluorides in various buf fer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of beta-D-mannopyranosyl fluor ide in gram quantities is given. (C) 2000 Elsevier Science Ltd. All rights reserved.