HEPATIC ADENINE-NUCLEOTIDES AND MICROSOMAL CHOLESTEROL 7-ALPHA-HYDROXYLASE ACTIVITY IN THE OBSTRUCTED AND FREELY DRAINING LOBES OF THE LIVER AFTER SELECTIVE BILE-DUCT OBSTRUCTION
K. Chijiiwa et al., HEPATIC ADENINE-NUCLEOTIDES AND MICROSOMAL CHOLESTEROL 7-ALPHA-HYDROXYLASE ACTIVITY IN THE OBSTRUCTED AND FREELY DRAINING LOBES OF THE LIVER AFTER SELECTIVE BILE-DUCT OBSTRUCTION, Research in experimental medicine, 197(1), 1997, pp. 13-22
Background: The effect of selective bile duct obstruction (SBDO) on he
patic reserve function of the bile duct obstructed (BDO) and nonobstru
cted freely draining (FD) lobes of the liver is obscure. Methods: The
bile duct branches draining from the left lateral and median lobes of
the liver were ligated for 4 and 10 days in rats, and hepatic reserve
functions in BDO and FD lobes were assessed by microsomal cholesterol
7 alpha-hydroxylase activities and by hepatic adenine nucleotide and e
nergy charge levels. The values were compared with those in sham-opera
ted control liver. Cholesterol 7 alpha-hydroxylase activities were det
ermined by gas-liquid chromatography-mass spectrometry, and hepatic ad
enosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine
monophosphate (AMP) levels with high-pressure liquid chromatography.
Results: The histological examination of the BDO lobes showed prolifer
ation and formation of new bile ductules and fibrous connective tissue
linking portal areas. Microsomal cholesterol 7 alpha-hydroxylase acti
vities, hepatic energy charge and adenine nucleotide levels did not di
ffer between FD and BDO lobes, and the values were similar to those in
the sham-operated liver. Conclusions: Selective bile duct obstruction
shows no adverse effects on microsomal and mitochondrial functions in
either BDO or FD lobes of the liver.