Cloning, characterization and mapping of human SPIN to human chromosome 9q22.1-22.3

Spindle is a specific substructure in cell during meiosis and gamete maturi ty division and its structure and function directly influence the developme nt, differentiation, growth and reproduction of individuals. Recent researc hes also show that mouse Mos/MAPK pathway is tightly related to spindlin ph osphorylation. We report that ( i) a novel gene encoding a putative human s pindlin (SPIN) has been cloned, which is highly homologous to mouse Spin; ( ii) tissue expression pattern of SPIN, shows that SPIN is highly expressed as a 4.8 kb transcript in ovary, testis, heart, brain, kidney, pancreas, pl acenta and spleen, also with a specific 2.0 kb transcript in testis, but th ere is no hybridization band in thymus and small intestine, and (iii) SPIN has been mapped to human chromosome 9q22.1-22.3 by Radiation Hybrid Mapping . This novel gene was registered in GenBank as SPIN with accession number A F087864.