Analysis of the compounds in the synthesis mixtures of hydroxy acids by a gradient programmed RP-HPLC with UV/vis and RI detectors

Authors
Koivusalmi, E Saarela, T Root, A Karvinen, E
Citation
E. Koivusalmi et al., Analysis of the compounds in the synthesis mixtures of hydroxy acids by a gradient programmed RP-HPLC with UV/vis and RI detectors, CHROMATOGR, 52(1-2), 2000, pp. 27-32
Citations number
22
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
52
Issue
1-2
Year of publication
2000
Pages
27 - 32
Database
ISI
SICI code
0009-5893(200007)52:1-2<27:AOTCIT>2.0.ZU;2-6
Abstract
A gradient programmed reversed phase high-performance liquid chromatographi c (RP-HPLC) method is described for the quantitative determination of some aldehydes, hydroxy aldehydes, unsaturated aldehydes, hydroxy carboxylic aci ds, carboxylic acids, alcohols and polyols using an ultraviolet and a refra ctive index detector in series. The sample matrices are synthesis mixtures of hydroxy carboxylic acids. The structures of the hydroxy aldehyde interme diates are determined by C-13 NMR and the influence of sample preparation o n analysis results is discussed. The limits of detection and the precision of the method are evaluated.