Determination of histamine in part wines and grape juices by ion-pair extraction and stable isotope dilution GC-MS

An isotope dilution, GC-MS method for the quantitation of histamine in wine s and grape juices was assayed. This method includes isolation of histamine and its deuterated analogue [alpha,alpha,beta,beta-H-2(4)]histamine, used as the labelled internal standard, by ion-pair extraction with bis-2-ethylh exylphosphate, conversion to the volatile derivative: tris-pentafluorobenzy l-histamine, capillary GC separation and measurement of the abundance ratio of the (M-181)(+) ions from labelled and unlabelled derivatives. The method was successfully applied to the determination of histamine in se veral samples of Port wine, red and white table wine and grape juice. Data are given on detectability, linearity, repeatability and recovery.