M. Aufderheide et U. Mohr, CULTEX - an alternative technique for cultivation and exposure of cells ofthe respiratory tract to airborne pollutants at the air/liquid interface, EXP TOX PAT, 52(3), 2000, pp. 265-270
The assessment of cytotoxicity of air contaminants such as gaseous or parti
culate compounds and complex mixtures has traditionally involved animal exp
eriments, due to the difficulties in exposing cell cultures directly to the
se substances.
New cultivation and exposure techniques enhance the efficiency of in vitro
studies, as demonstrated by a new experimental system called CULTEX1 which
allows direct exposure of cells at the air/liquid interface. In this case,
human bronchial epithelial cells are cultivated on porous transwell membran
es in a device allowing intermittent medium supply. The medium is pumped in
to a special modular culture unit through the transwell membrane supporting
the cells. At certain time intervals, the medium is completely removed and
the cells can be maintained and exposed at the air/liquid interface until
the next medium supply without loss of viability. In comparison to conventi
onal submersed culture conditions, the cells have been grown on transwell m
embranes using the new pulse submersion technique. There are no deleterious
effects on cell viability due to the direct exposure to airborne pollutant
s. Thus, the introduction of these new cultivation and exposure techniques
offers new testing strategies for the toxicological evaluation of inhalable
soluble and inert substances as well as complex mixtures.