Establishment of SSEA-1-and Oct-4-expressing rat embryonic stem-like cell lines and effects of cytokines of the IL-6 family on clonal growth

Citation
S. Vassilieva et al., Establishment of SSEA-1-and Oct-4-expressing rat embryonic stem-like cell lines and effects of cytokines of the IL-6 family on clonal growth, EXP CELL RE, 258(2), 2000, pp. 361-373
Citations number
80
Categorie Soggetti
Cell & Developmental Biology
Journal title
EXPERIMENTAL CELL RESEARCH
ISSN journal
00144827 → ACNP
Volume
258
Issue
2
Year of publication
2000
Pages
361 - 373
Database
ISI
SICI code
0014-4827(20000801)258:2<361:EOSORE>2.0.ZU;2-N
Abstract
Here, we demonstrate long-term cultivation of alkaline phosphatase-positive rat embryonic stem-like (RES) cell lines. RES cells were characterized by their typical growth in highly compacted cell clusters, which were found to be sensitive against enzymatic dissociation. RES cells expressed stage-spe cific embryonic antigen-1 (SSEA-1) and transcription factor Oct-4, but Oct- 4 mRNA was detected at lower levels compared to mouse ES cells. Once establ ished to tissue culture, RES cells were able to grow in the absence of feed er cells under clonal conditions. Cytokines: of the interleukin-g family kn own to maintain the undifferentiated state of mouse ES cells were comparati vely analyzed for their capacity to maintain the undifferentiated growth of two cell lines, RES-1 and RES-15, in a clonal assay. Rat ciliary neurotrop hic factor (rCNTF), human oncostatin M (hOSM), and interleukin-6 and solubl e interleukin-6 receptor (IL-6/sIL-6R) were found to support clonal growth of RES cells, but the cytokines did not reach the efficiency of the colony forming ability of leukemia inhibitory factor (LIF). When RES-1 and RES-15 cells were cultivated without feeder cells, SSEA-1 expression was maintaine d after clonal growth in the presence of LIF and LIF + rCNTF, respectively. Oct-4 mRNA was significantly detected in RES-15 cells when cultivated in t he absence of feeder cells in media substituted by LIF and/or IL-6/sIL-6R, as well as without cytokines. In summary, rat embryonic stem-like cell line s could be established from rat blastocysts and were able to proliferate as undifferentiated alkaline phosphatase-positive cells. Embryonal stem cell properties, such as SSEA-1 and Oct-4 expression, were maintained by members of the IL-6 family of cytokines, but most significantly by LIF. (C) 2000 A cademic Press.