Wide crosses were made to identify new cytoplasmic male sterility (CMS
) systems in faba beans, based on the interaction of cytoplasm with re
storer and maintainer alleles. A total of 330 F-1 hybrids were produce
d in both reciprocal forms. Male sterile segregates were observed in o
ne reciprocal version in the F-2 generation of six crosses. Two of the
se crosses with female parents originating from Afghanistan and Egypt
expressed stable male sterility in subsequent backcross generations. B
ased on the female parents of the two crosses, these two CMS systems w
ere designated CMS 199 and CMS 297. CMS 199 was more stable than CMS 2
97 during backcross generations and across different environments. Mai
ntainer and restorer lines for both CMS systems were identified. Lower
expression of male sterility occurred in CMS 297 in the greenhouse du
ring the winter generations than in isolation cages during the summer
generations, which may be utilized to maintain male sterile lines by s
elfing. Regarding practical applications, the CMS 199 shows great prom
ise for hybrid breeding in faba beans.