Balance experiments on female pigs comprising two stages of growth (me
an body weights 51 kg and 83 kg) were conducted to estimate the minimu
m crude protein (CP) level allowing maximum rate of protein accretion
in amino acid-supplemented, wheat and barley-based diets. In Experimen
t 1, dietary CP was gradually decreased from 210 g/kg and 178 g/kg to
123 g/kg and 110 g/kg for Stage 1 and Stage 2, respectively while the
concentrations of lysine, threonine, methionine + cystine and tryptoph
an were kept constant, The critical CP level below which N retention s
tarted to decrease was estimated to be 157 g/kg and 142 g/kg for Stage
1 and Stage 2, respectively. CP levels corresponding to the breakpoin
ts in N excretion and plasma urea concentration were slightly lower th
an those found for N retention. Reducing dietary CP to minimum concent
ration for maximum N retention resulted in a decrease in total N exret
ion by 0.4 and 0.32 for Stage 1 and Stage 2, respectively. In Experime
nt 2, methionine and tryptophan supplements were successively deleted
from diets with the minimized CP contents to study the degree of their
deficiency. The results suggested that methionine was the third-limit
ing amino acid while tryptophan was not limiting or was co-limiting wi
th other amino acid(s). It is concluded that dietary CP and N excretio
n can be substantially reduced without affecting the rate of protein d
eposition as long as the diets are supplemented with lysine, threonine
and methionine.