A new coagulase-negative and novobiocin-resistant species of the genus Stap
hylococcus, Staphylococcus fleurettii, isolated from raw-milk cheeses, is d
escribed. This species is differentiated from the other novobiocin-resistan
t staphylococci on the basis of ribotype and intergenic transcribed spacer
patterns, DNA-DNA reassociation reactions, cell wall composition and phenot
ypic characteristics. S. fleurettii could be distinguished by its oxidase a
ctivity, by its ability to produce acid aerobically from D-trehalose, D-man
nose, D-turanose and maltose and by its inabililty to produce acid from D-c
ellobiose. The type strain of S. fleurettii is CIP 106114(T) (= DSM 13212(T
)).