Denitrovibrio acetiphilus, a novel genus and species of dissimilatory nitrate-reducing bacterium isolated from an oil reservoir model column

A novel dissimilatory, nitrate-reducing bacterium, designated strain N2460( T), was isolated from an oil reservoir model column. Strain N2460(T) is a m esophilic, obligately anaerobic, marine, Gram-negative bacterium. The cells are vibrio-shaped and motile by a bipolar flagellum. Strain N2460(T) reduc es nitrate to ammonia in a mineral medium supplied by acetate. The presence of a 2-oxoglutarate dehydrogenase activity indicates that acetate is oxidi zed via the citric acid cycle. No growth is obtained on formate, higher fat ty acids, malate, fumarate, benzoate, alcohols, sugar, yeast extract, crude oil, alkanes, proline, hydrogen, sulfur or thiosulfate with nitrate as ele ctron acceptor. Oxygen, sulfate, thiosulfate and sulfur are not utilized as alternative electron accepters. Strain N2460(T) grows fermentatively on fu marate, but not on pyruvate. The G+C content of the DNA is 42.6 mol%. 16S r RNA gene analysis shows that strain N2460(T) belongs to the Bacteria and th at the closest relative is 'Geovibrio ferrireducens' (sequence similarity 8 6.9%). On the basis of phylogenetic as well as phenotypic data, it is propo sed that strain N2460(T) represents the type strain of a new genus and spec ies, Denitrovikrio acetiphilus gen. nov., sp. nov.