SUPPRESSIVE EFFECTS OF TRITERPENOIDS ON CINC-1 INDUCTION IN INTERLEUKIN-1-BETA-STIMULATED RAT FIBROBLAST NRK-49F CELLS

CINC-1 is a member of chemokine family with chemotactic and activating properties to neutrophils. CINC-1 induction in IL-1 beta-stimulated r at fibroblast NRK-49F cells was quantitated by a sensitive ELISA. CINC -1 production was increased up to 135 ng/ml from basal 2-6 ng/ml by st imulation with IL-1 beta. Steroidal anti-inflammatory drugs including dexamethasone and prednisolone exhibited potent suppressive effects on IL-1 beta-induced CINC-1 production. Among 39 kinds of natural triter penoids tested, acacigenin B exhibited the highest suppressive effects with about 10 mu M to be 50% of inhibition on the CINC-1 induction. T he suppressive potency of acacigenin B on IL-1 beta-induced CINC-1 pro duction was about 10-fold less than that of the steroidal anti-inflamm atory drugs.