Discovery of novel ansamycins possessing potent inhibitory activity in a cell-based oncostatin M signalling assay

Authors
Stead, P Latif, S Blackaby, AP Sidebottom, PJ Deakin, A Taylor, NL Life, P Spaull, J Burrell, F Jones, R Lewis, J Davidson, I Mander, T
Citation
P. Stead et al., Discovery of novel ansamycins possessing potent inhibitory activity in a cell-based oncostatin M signalling assay, J ANTIBIOT, 53(7), 2000, pp. 657-663
Citations number
16
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF ANTIBIOTICS
ISSN journal
00218820 → ACNP
Volume
53
Issue
7
Year of publication
2000
Pages
657 - 663
Database
ISI
SICI code
0021-8820(200007)53:7<657:DONAPP>2.0.ZU;2-4
Abstract
We describe the isolation and characterisation of novel non-benzoquinone an samycin metabolites related to geldanamycin from a culture of Streptomyces sp. S6699. The compounds possess potent inhibitory activity in a cell-based assay measuring inhibition of oncostatin M signalling in a reporter cell l ine utilising a secreted placental alkaline phosphatase (sPAP) readout. In this paper we report the isolation and structure elucidation of the compoun ds and describe some of their biological properties.